Interestingly, retroviralmediated suppression of AHI1 also reduced autocrine production of IL2 (40%), IL4 (30%) and, more significantly, of TNF (85%) in Hut78 cells as compared to control cells, as evaluated by QRTPCR (Figure 2a). However, knocking down AHI1 expression had no effect on the autocrine production IFN when evaluated using the same detection method (data not shown). A significant reduction in autocrine production of TNF in several AHIsh4transduced cell lines, when compared to control cells, was further detected when measured by ELISA (5.53.5 vs 645 pg/ml, PFigure 2b).Dessert and beverage will be provided by the volunteer hostess Dorothy Clowson. Monday through Friday. Noon Kiwanis meets at Pepper's Grill.

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